Yangzhong Liu
1、Investigation of metalloproteins in the mechanism of metallodrugs
Investigation of the protein interaction of metallodrugs using NMR and MS spectroscopies in order to elucidate the fundamental physical chemistry processes, the selectivity of metallodrugs to various proteins, and the influence of protein interaction on the efficacy of drugs.
Representative studies:
Tetrathiomolybdate Induces Dimerization of the Metal-binding Domain of ATPase and Inhibits Platination of the Protein,
Nature Communications, 2019, 10, 186
A dual functional ruthenium arene complex induces differentiation and apoptosis of acute promyelocytic leukemia cells
Chem. Sci., 2019, 10, 9721-9728.
2、Design and targeted delivery of metallodrugs
Designing novel Pt(IV) anticancer prodrugs based on the mechanism studies; construction of nanobody-drug conjugates in order to enhance the drug accumulation and reduce adverse effects of the drugs; Using functional vectors to achieve synergistic effects on the tumor therapy.
Representative studies:
Stimuli-Responsive Manganese Single-Atom Nanozyme for Tumor Therapy via Integrated Cascade Reactions,
Angew. Chem. Int. Ed. 2021, 60(17), 9480-9488. [Link]
Manganese-deposited iron oxide promotes tumor-responsive ferroptosis that synergizes the apoptosis of cisplatin.
Theranostics, 2021, 11(11):5418-5429 [Link]
Changlin Tian
Structure, dynamic and functional mechanism investigation of important drug target membrane proteins, R&D and method development of high-field magnetic resonance equipment, Chemical synthesis of post-translational modified histones and nucleosome assembly.
1、Structural and functional study of ion channels and G protein-coupled receptors (GPCRs)
Representative studies：
(1). Structural insights into human acid sensing ion channel inhibition by snake toxin mambalgin1
Chem Commun 50(44): 5837-5839 (2014).
Chem Commun 51(38):8153-8156 (2015).
Cell Discovery 4:27 (2018).
Elife 9:e57096 (2020).
(2). Structural basis of human alpha7 nicotinic acetylcholine receptor activation
Cell Research 31(6):713-716 (2021)
(3). Structural mechanism of cooperative activation of the human calcium-sensing receptor by Ca2+ ions and L-tryptophan
Cell Research 31 (2021) 383-394(2021)
(4). Different Conformational Responses of the β2-Adrenergic Receptor-Gs Complex upon Binding of the Partial Agonist Salbutamol or the Full Agonist Isoprenaline
National Science Review.. doi.org/10.1093/nsr/nwaa284. (2020)
Chem Commun (Camb) Jun 25;56(51):6941-6944. (2020)
Cell Discovery, Jul 7;6:45. (2020)
2、Chemically synthesized of post-translationally modified proteins and deciphering the epigenetic mechanism of post-translationally modified histone.
Representative studies：
(1).New methods for chemical synthesis of post-translational modified proteins
J Am Chem Soc 138, 3553-3561(2016)
Angew Chem Int Ed 56, 2744-2748(2017).
Nature Protocols 12, 2554-2569(2017).
J Am Chem Soc 142, 8790-8799(2020).
(2).Epigenetic mechanism of histone post-translational modification
Chembiochem 18, 176-180 (2017).
Cell Research 28, 257-260(2018).
Chem Comm 55, 12639-12642(2019).
(3).Methodological development and application research of protein dynamic characteristics based on nuclear magnetic resonance (NMR), electron spin resonance (ESR), fluorescence (FLIM) and other spectroscopy methods.
Representative studies：
(1). Electron Transfer mechanism of yeast NADH dehydrogenase Ndi1
Nature 491, 478-482(2012).
PCCP 19, 4849-4854(2017).
(2). Electron Transfer mechanism of respiratory chain of Mycobacteria
Science 362, eaat8923(2018).
Nat Commun 11, 4245(2020).
PNAS 118, e2022308118(2021).
(3). Catalytic and Electron Transfer(ET) mechanism of biological enzymatic reactions and transition metal-catalyzed organic reactions.
ACS Catalysis 11, 5628-5635(2021).
CCS Chemistry 3, 727-737(2021).
Chem Sci 8, 5067-5077(2017).
Angew Chem Int Ed Engl 58, 16480-16484(2019).
Chin J Chem, 37, 11-18(2019).
Jing Shi
1、Theoretical Studies onOrganic Reactions
Mechanism and Origin of the Stereoselectivity in the Palladium-Catalyzed trans Hydroboration of Internal 1,3-Enynes with an Azaborine-Based Chemistry - A European Journal 2018 , 24(1)：178-186.
Mechanistic Study of Copper-Catalyzed Decarboxylative C–N Cross-Coupling with Hypervalent Iodine Oxidant Organometallics, 2017,36:2081-2081.
2、Chemical Synthesis and Modification of Peptide Drugs and Probes
Chemical synthesis and biological activity of peptides incorporating an ether bridge as a surrogate for a disulfide bond Chem Sci, 2020, 11: 7927-7932.
An activity-based probe developed by a sequential dehydroalanine formation strategy targets HECT E3 ubiquitin ligases Chemical Communications 2019, 55, 7109-7112.
Chemical Synthesis of Natural Polyubiquitin Chains through Auxiliary-Mediated Ligation of an Expressed Ubiquitin Isomer Organic Letters 2018, 20, 329-332.